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Human CLIC5 Recombinant Protein (N-His) (HD391012)

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Aperçu
Numéro de catalogueHD391012
Description
Recombinant Human CLIC5 Protein, N-His (HD391012) expressed in E. coli, spanning Pro173-Ser410. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ9NZA1
Longueur de protéinePro173-Ser410
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 29.16 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsCLIC5, Chloride intracellular channel protein 5, EC:1.8.-.-, Glutaredoxin-like oxidoreductase CLIC5
Contexte

Chloride intracellular channel protein 5 is a ~46 kDa protein. In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Can insert into membranes and form non-selective ion channels almost equally permeable to Na(+), K(+) and Cl(-). Required for normal hearing. It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti. May play a role in the regulation of transepithelial ion absorption and secretion.

1. Berryman, M. et al. (2004) The Journal of biological chemistry 279, 34794-801. PMID: 15184393
2. Singh, H. et al. (2007) The FEBS journal 274, 6306-16. PMID: 18028448
3. Seco, CZ. et al. (2015) European journal of human genetics : EJHG 23, 189-94. PMID: 24781754
4. Wegner, B. et al. (2010) American journal of physiology. Renal physiology 298, F1492-503. PMID: 20335315
Note For research use only
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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