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| カタログ番号 | HB822012 |
|---|---|
| 説明 |
Recombinant Human CNR1/CB1 Protein, N-GST & C-His (HB822012) expressed in E. coli, spanning Lys2-Gln116. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P21554 |
| タンパク質長 | Lys2-Gln116 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 41.19 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CNR1, CB1, CANN6, CB-R, CNR, Cannabinoid receptor 1 |
| 背景 | Cannabinoid receptor 1 (CNR1/CB1) is a ~52 kDa protein. G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta(9)-tetrahydrocannabinol (THC). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP. In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses. 1. Ryberg, E. et al. (2005) FEBS letters 579, 259-64. PMID: 15620723 2. Hua, T. et al. (2016) Cell 167, 750-762.e14. PMID: 27768894 3. Shao, Z. et al. (2016) Nature 540, 602-606. PMID: 27851727 4. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350 5. Gérard, CM. et al. (1991) The Biochemical journal 279 ( Pt 1), 129-34. PMID: 1718258 6. Oddi, S. et al. (2012) British journal of pharmacology 165, 2635-51. PMID: 21895628 7. Niehaus, JL. et al. (2007) Molecular pharmacology 72, 1557-66. PMID: 17895407 8. Jourdan, T. et al. (2013) Nature medicine 19, 1132-40. PMID: 23955712 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CNR1/CB1 Protein, N-GST & C-His
Detected by SAA1274 (Catalog No: HB822013) in indirect ELISA.
Detected by 5D12 (Catalog No: HB822010) in indirect ELISA.
Detected by 6B7 (Catalog No: HB822030) in indirect ELISA.
Detected by 5G11 (Catalog No: HB822020) in indirect ELISA.
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