Human CPEB4/CPE-BP4 Recombinant Protein (N-His) (HA414012)

Name: Human CPEB4/CPE-BP4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA414012
Price: 298 USD
Availability: InStock

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Numéro de catalogue	HA414012
Description	Recombinant Human CPEB4/CPE-BP4 Protein, N-His (HA414012) expressed in E. coli, spanning Glu468-Ala705. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q17RY0
Longueur de protéine	Glu468-Ala705
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	29.02 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Cytoplasmic polyadenylation element-binding protein 4, CPE-BP4, CPE-binding protein 4, hCPEB-4, CPEB4, KIAA1673
Contexte	Cytoplasmic polyadenylation element-binding protein 4 (CPEB4/CPE-BP4) is a ~80 kDa protein. Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism. Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited. Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation. Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT. 1. Afroz, T. et al. (2014) Genes & development 28, 1498-514. PMID: 24990967 2. Giangarrà, V. et al. (2015) PloS one 10, e0138794. PMID: 26398195 3. Ortiz-Zapater, E. et al. (2011) Nature medicine 18, 83-90. PMID: 22138752 4. Pérez-Guijarro, E. et al. (2016) Nature communications 7, 13418. PMID: 27857118
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CPEB4/CPE-BP4 Protein, N-His

Reférences

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Human CPEB4/CPE-BP4 Recombinant Protein (N-His).pdf

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Formula

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