Human CPT1A Recombinant Protein (N-His) (HW523012)

Name: Human CPT1A Recombinant Protein (N-His)
Brand: abinScience
SKU: HW523012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HW523012
Description	Recombinant Human CPT1A Protein, N-His (HW523012) expressed in E. coli, spanning Gln406-Thr745. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P50416
Longueur de protéine	Gln406-Thr745
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	40.74 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CPT1, CPTI-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT1A, CPT I, Carnitine palmitoyltransferase 1A, CPT1-L, Carnitine O-palmitoyltransferase 1, liver isoform
Contexte	Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) is a ~88 kDa protein. Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Also possesses a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity. Plays an important role in hepatic triglyceride metabolism. Also plays a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression. Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation. 1. Prip-Buus, C. et al. (2001) Molecular genetics and metabolism 73, 46-54. PMID: 11350182 2. Gobin, S. et al. (2003) The Journal of biological chemistry 278, 50428-34. PMID: 14517221 3. Wolfgang, MJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 7282-7. PMID: 16651524 4. IJlst, L. et al. (1998) The Journal of clinical investigation 102, 527-31. PMID: 9691089 5. Kurmi, K. et al. (2018) Cell reports 22, 1365-1373. PMID: 29425493 6. Zhang, G. et al. (2023) Molecular cell 83, 4370-4385.e9. PMID: 38016475
Note	For research use only.

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SDS-PAGE for Human CPT1A Recombinant Protein (N-His)

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Human CPT1A Recombinant Protein (N-His).pdf

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