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Human CTSC Recombinant Protein (N-His) (HW671012)

Human CTSC Recombinant Protein (N-His)
Human CTSC Recombinant Protein (N-His)
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  • 50ug
  • 100ug
  • 1mg
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  • SDS

Aperçu
Numéro de catalogueHW671012
Description
Recombinant Human CTSC Protein, N-His (HW671012) expressed in E. coli, spanning Leu231-Leu463. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsP53634
Longueur de protéineLeu231-Leu463
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 28.33 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsDipeptidyl peptidase 1, Dipeptidyl peptidase I exclusion domain chain, DPPI, Cathepsin J, Dipeptidyl transferase, Dipeptidyl peptidase I heavy chain, CTSC, Dipeptidyl peptidase I, Dipeptidyl peptidase I light chain, CPPI, Cathepsin C, DPP-I
Contexte

Dipeptidyl peptidase 1 (CTSC) is a ~51 kDa protein. Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase.

1. McGuire, MJ. et al. (1992) Archives of biochemistry and biophysics 295, 280-8. PMID: 1586157
2. McGuire, MJ. et al. (1993) The Journal of biological chemistry 268, 2458-67. PMID: 8428921
Note For research use only.
Images
  • Human CTSC Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human CTSC Protein, N-His

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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