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| カタログ番号 | HY284012 |
|---|---|
| 説明 |
Recombinant Human CTSG Protein, N-GST (HY284012) expressed in E. coli, spanning Ile21-Leu255. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P08311 |
| タンパク質長 | Ile21-Leu255 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 53.58 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Cathepsin G, CG, CTSG |
| 背景 | Cathepsin G (CTSG) is a ~28 kDa protein. Serine protease with trypsin- and chymotrypsin-like specificity. Also displays antibacterial activity against Gram-negative and Gram-positive bacteria independent of its protease activity. Prefers Phe and Tyr residues in the P1 position of substrates but also cleaves efficiently after Trp and Leu. Shows a preference for negatively charged amino acids in the P2' position and for aliphatic amino acids both upstream and downstream of the cleavage site. Required for recruitment and activation of platelets which is mediated by the F2RL3/PAR4 platelet receptor. 1. Thorpe, M. et al. (2018) PloS one 13, e0195077. PMID: 29652924 2. Avril, LE. et al. (1994) FEBS letters 345, 81-6. PMID: 8194606 3. Bangalore, N. et al. (1990) The Journal of biological chemistry 265, 13584-8. PMID: 2116408 4. Alford, CE. et al. (1990) Journal of general microbiology 136, 997-100. PMID: 2117044 5. Sambrano, GR. et al. (2000) The Journal of biological chemistry 275, 6819-23. PMID: 10702240 6. Selak, MA. et al. (1988) The Biochemical journal 251, 293-9. PMID: 3390156 7. Hase-Yamazaki, T. et al. (1995) Cellular immunology 160, 24-32. PMID: 7842483 8. Yamazaki, T. et al. (1997) Journal of leukocyte biology 61, 73-9. PMID: 9000539 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human CTSG Protein, N-GST
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