中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HD954012 |
|---|---|
| 説明 |
Recombinant Human CYP27A1 Protein, N-His (HD954012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q02318 |
| タンパク質長 | Arg52-Cys531 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CYP27, CYP27A1, Cytochrome P-450C27/25, Cytochrome P450 27, EC:1.14.15.15, Sterol 26-hydroxylase, mitochondrial, Sterol 27-hydroxylase, Vitamin D(3) 25-hydroxylase |
| 背景 | Sterol 26-hydroxylase, mitochondrial is a ~60 kDa protein. Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism. Plays a role in cholestanol metabolism in the cerebellum. 1. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124 2. Heo, GY. et al. (2011) Journal of lipid research 52, 1117-1127. PMID: 21411718 3. Mast, N. et al. (2017) The Journal of biological chemistry 292, 4913-4924. PMID: 28190002 4. Pikuleva, IA. et al. (1998) The Journal of biological chemistry 273, 18153-60. PMID: 9660774 5. Pikuleva, IA. et al. (2001) Biochemistry 40, 7621-9. PMID: 11412116 6. Cali, JJ. et al. (1991) The Journal of biological chemistry 266, 7774-8. PMID: 1708392 7. Cali, JJ. et al. (1991) The Journal of biological chemistry 266, 7779-83. PMID: 2019602 8. Kim, KS. et al. (1994) Journal of lipid research 35, 1031-9. PMID: 7915755 9. Chen, W. et al. (1997) Journal of lipid research 38, 870-9. PMID: 9186905 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
