Human CYP2C9 Recombinant Protein (N-His) (HB549012)

Name: Human CYP2C9 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB549012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HB549012
Описание	Recombinant Human CYP2C9 Protein, N-His (HB549012) expressed in E. coli, spanning Gly40-Val490. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P11712
Длина белка	Gly40-Val490
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	53.77 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Cytochrome P450 2C9; 1.14.14.1; (R)-limonene 6-monooxygenase; 1.14.14.53; (S)-limonene 6-monooxygenase; 1.14.14.51; (S)-limonene 7-monooxygenase; 1.14.14.52; CYPIIC9; Cholesterol 25-hydroxylase; Cytochrome P-450MP; Cytochrome P450 MP-4; Cytochrome P450 MP-8; Cytochrome P450 PB-1; S-mephenytoin 4-hydroxylase; CYP2C9; CYP2C10
Фон	Cytochrome P450 2C9 is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. 1. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317 2. Barbosa-Sicard, E. et al. (2005) Biochemical and biophysical research communications 329, 1275-81. PMID: 15766564 3. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576 4. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599 5. Zeldin, DC. et al. (1995) Archives of biochemistry and biophysics 322, 76-86. PMID: 7574697 6. Bylund, J. et al. (1998) The Journal of pharmacology and experimental therapeutics 284, 51-60. PMID: 9435160 7. Bylund, J. et al. (1998) Analytical biochemistry 265, 55-68. PMID: 9866708 8. Miyazawa, M. et al. (2002) Drug metabolism and disposition: the biological fate of chemicals 30, 602-7. PMID: 11950794
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human CYP2C9 Protein, N-His

Ссылки

Даташит

Human CYP2C9 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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