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Human CYP2J2 Recombinant Protein (N-His) (HD745012)

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  • 1mg
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Vista general
Número de catálogoHD745012
Descripción
Recombinant Human CYP2J2 Protein, N-His (HD745012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoP51589
Longitud de proteínaGly54-Val502
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosAlbendazole monooxygenase (hydroxylating), Albendazole monooxygenase (sulfoxide-forming), Arachidonic acid epoxygenase, CYP2J2, CYPIIJ2, Cytochrome P450 2J2, EC:1.14.14.-, EC:1.14.14.73, EC:1.14.14.74, EC:5.4.4.7, Hydroperoxy icosatetraenoate isomerase
Fondo

Cytochrome P450 2J2 is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of PUFA. Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools. In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites.

1. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576
2. Wu, S. et al. (1996) The Journal of biological chemistry 271, 3460-8. PMID: 8631948
3. Chawengsub, Y. et al. (2009) The Journal of biological chemistry 284, 31280-90. PMID: 19737933
4. Lee, CA. et al. (2010) Drug metabolism and disposition: the biological fate of chemicals 38, 347-56. PMID: 19923256
5. Wu, Z. et al. (2013) Antimicrobial agents and chemotherapy 57, 5448-56. PMID: 23959307
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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