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Обзор
Каталожный номерHD745012
Описание
Recombinant Human CYP2J2 Protein, N-His (HD745012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаP51589
Длина белкаGly54-Val502
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияAlbendazole monooxygenase (hydroxylating), Albendazole monooxygenase (sulfoxide-forming), Arachidonic acid epoxygenase, CYP2J2, CYPIIJ2, Cytochrome P450 2J2, EC:1.14.14.-, EC:1.14.14.73, EC:1.14.14.74, EC:5.4.4.7, Hydroperoxy icosatetraenoate isomerase
Фон

Cytochrome P450 2J2 is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) in the cardiovascular system. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of PUFA. Converts arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EpETrE), likely playing a major role in the epoxidation of endogenous cardiac arachidonic acid pools. In endothelial cells, participates in eicosanoids metabolism by converting hydroperoxide species into hydroxy epoxy metabolites.

1. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576
2. Wu, S. et al. (1996) The Journal of biological chemistry 271, 3460-8. PMID: 8631948
3. Chawengsub, Y. et al. (2009) The Journal of biological chemistry 284, 31280-90. PMID: 19737933
4. Lee, CA. et al. (2010) Drug metabolism and disposition: the biological fate of chemicals 38, 347-56. PMID: 19923256
5. Wu, Z. et al. (2013) Antimicrobial agents and chemotherapy 57, 5448-56. PMID: 23959307
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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