

| Numéro de catalogue | HD360012 |
|---|---|
| Description |
Recombinant Human CYP2R1 Protein, N-His (HD360012) expressed in E. coli, spanning Met37-Arg501. Purity: >90% by SDS-PAGE.
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | Q6VVX0 |
| Longueur de protéine | Met37-Arg501 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Homo sapiens (Human) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Masse moléculaire prédite | 55.46 kDa |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | CYP2R1, Cytochrome P450 2R1, EC:1.14.14.24, Vitamin D 25-hydroxylase |
| Contexte | Vitamin D 25-hydroxylase is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). 1. Cheng, JB. et al. (2003) The Journal of biological chemistry 278, 38084-93. PMID: 12867411 2. Shinkyo, R. et al. (2004) Biochemical and biophysical research communications 324, 451-7. PMID: 15465040 3. Strushkevich, N. et al. (2008) Journal of molecular biology 380, 95-106. PMID: 18511070 |
| Note | For research use only |
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