中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HD122012 |
|---|---|
| 説明 |
Recombinant Human CYP4F2 Protein, N-His (HD122012) expressed in E. coli, spanning Gly62-Ser520. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P78329 |
| タンパク質長 | Gly62-Ser520 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 54.89 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, Arachidonic acid omega-hydroxylase, CYP4F2, CYPIVF2, Cytochrome P450 4F2, Cytochrome P450-LTB-omega, Docosahexaenoic acid omega-hydroxylase, EC:1.14.14.1, EC:1.14.14.78, EC:1.14.14.79, EC:1.14.14.94, Leukotriene-B(4) 20-monooxygenase 1, Leukotriene-B(4) omega-hydroxylase 1, Phylloquinone omega-hydroxylase CYP4F2 |
| 背景 | Cytochrome P450 4F2 is a ~59 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, eicosanoids and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of long- and very long-chain fatty acids. Displays high omega-hydroxylase activity toward polyunsaturated fatty acids (PUFAs). Participates in the conversion of arachidonic acid to omega-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure. 1. Lasker, JM. et al. (2000) The Journal of biological chemistry 275, 4118-26. PMID: 10660572 2. Kikuta, Y. et al. (2000) Journal of biochemistry 127, 1047-52. PMID: 10833273 3. Sontag, TJ. et al. (2002) The Journal of biological chemistry 277, 25290-6. PMID: 11997390 4. Stec, DE. et al. (2007) Physiological genomics 30, 74-81. PMID: 17341693 5. Fava, C. et al. (2008) Hypertension (Dallas, Tex. : 1979) 52, 373-80. PMID: 18574070 6. Fer, M. et al. (2008) Journal of lipid research 49, 2379-89. PMID: 18577768 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
