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| カタログ番号 | HS873022 |
|---|---|
| 説明 |
Recombinant Human DDB1 Protein, N-His (HS873022) expressed in E. coli, spanning Met1-His1140. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q16531 |
| タンパク質長 | Met1-His1140 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 129.13 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DDB p127 subunit, DDB1, DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, UV-damaged DNA-binding protein 1, XAP-1, XAP1, XPCe, XPE-BF, XPE-binding factor, Xeroderma pigmentosum group E-complementing protein |
| 背景 | DNA damage-binding protein 1 is a ~126 kDa protein. Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. 1. Wertz, IE. et al. (2004) Science (New York, N.Y.) 303, 1371-4. PMID: 14739464 2. Hu, J. et al. (2004) Nature cell biology 6, 1003-9. PMID: 15448697 3. Kulaksiz, G. et al. (2005) Molecular and cellular biology 25, 9784-92. PMID: 16260596 4. Hu, J. et al. (2006) The Journal of biological chemistry 281, 3753-6. PMID: 16407242 5. Senga, T. et al. (2006) The Journal of biological chemistry 281, 6246-52. PMID: 16407252 6. Nishitani, H. et al. (2006) The EMBO journal 25, 1126-36. PMID: 16482215 7. Lovejoy, CA. et al. (2006) Molecular and cellular biology 26, 7977-90. PMID: 16940174 8. He, YJ. et al. (2006) Genes & development 20, 2949-54. PMID: 17079684 9. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626 |
| 注意事項 | For research use only |
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