Human DDO Recombinant Protein (N-His) (HV468012)

Name: Human DDO Recombinant Protein (N-His)
Brand: abinScience
SKU: HV468012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HV468012
Descripción	Recombinant Human DDO Protein, N-His (HV468012) expressed in E. coli, spanning Arg117-Val199. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q99489
Longitud de proteína	Arg117-Val199
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	39.69 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	DDO, D-aspartate oxidase, DASOX
Fondo	D-aspartate oxidase (DDO) is a ~37 kDa protein. Selectively catalyzes the oxidative deamination of acidic amino acids. Suppresses the level of D-aspartate in the brain, an amino acid that can act as an agonist for glutamate receptors. Protects the organism from the toxicity of D-amino acids. May also function in the intestine. 1. Van Veldhoven, PP. et al. (1991) Biochimica et biophysica acta 1073, 203-8. PMID: 1991137 2. Katane, M. et al. (2010) Biochimie 92, 1371-8. PMID: 20603179 3. Katane, M. et al. (2013) Journal of medicinal chemistry 56, 1894-907. PMID: 23391306 4. Katane, M. et al. (2015) Biological & pharmaceutical bulletin 38, 298-305. PMID: 25747990 5. Sacchi, S. et al. (2017) Scientific reports 7, 46288. PMID: 28393897 6. Nuzzo, T. et al. (2017) NPJ schizophrenia 3, 16. PMID: 28560262 7. Katane, M. et al. (2017) Biochimica et biophysica acta. Proteins and proteomics 1865, 1129-1140. PMID: 28629864 8. Katane, M. et al. (2018) Biochimica et biophysica acta. Proteins and proteomics 1866, 806-812. PMID: 29292239 9. Molla, G. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 1182-1197. PMID: 31914658 10. Puggioni, V. et al. (2020) Biochimica et biophysica acta. Proteins and proteomics 1868, 140472. PMID: 32553892
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human DDO Protein, N-His

Referencias

Datasheet

Human DDO Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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