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Human DDX17 Recombinant Protein (C-Strep) (HC205011)

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Aperçu
Numéro de catalogueHC205011
Description
Recombinant Human DDX17 Protein, C-Strep (HC205011) expressed in Insect Cells, spanning Gly111-Gly556. Purity: >90% by SDS-PAGE.
Highlights
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionInsect cells
Numéro d'accèsQ92841
Longueur de protéineGly111-Gly556
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 52.39 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsDDX17, DEAD box protein 17, DEAD box protein p72, DEAD box protein p82, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX17, RNA-dependent helicase p72
Contexte

Probable ATP-dependent RNA helicase DDX17 is a ~80 kDa protein. As an RNA helicase, unwinds RNA and alters RNA structures through ATP binding and hydrolysis. Involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. Regulates the alternative splicing of exons exhibiting specific features. For instance, promotes the inclusion of AC-rich alternative exons in CD44 transcripts. This function requires the RNA helicase activity.

1. Hönig, A. et al. (2002) Molecular and cellular biology 22, 5698-707. PMID: 12138182
2. Germann, S. et al. (2012) Oncogene 31, 4536-49. PMID: 22266867
3. Dardenne, E. et al. (2012) Nature structural & molecular biology 19, 1139-46. PMID: 23022728
4. Dardenne, E. et al. (2014) Cell reports 7, 1900-13. PMID: 24910439
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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