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| カタログ番号 | HF777012 |
|---|---|
| 説明 |
Recombinant Human DDX58 Protein, N-His (HF777012) expressed in E. coli, spanning Ile725-Lys925. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O95786 |
| タンパク質長 | Ile725-Lys925 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 25.87 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Probable ATP-dependent RNA helicase DDX58, Retinoic acid-inducible gene I protein, RIG-1, DEAD box protein 58, RLR-1, DDX58, Retinoic acid-inducible gene 1 protein, Antiviral innate immune response receptor RIG-I, RIG-I-like receptor 1, RIG-I |
| 背景 | Antiviral innate immune response receptor RIG-I (DDX58) is a ~106 kDa protein. Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines. Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments. The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms. Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons. Ligands include 5'-triphosphorylated ssRNAs and dsRNAs but also short dsRNAs (<1 kb in length). 1. Yoneyama, M. et al. (2004) Nature immunology 5, 730-7. PMID: 15208624 2. Sumpter, R. et al. (2005) Journal of virology 79, 2689-99. PMID: 15708988 3. Seth, RB. et al. (2005) Cell 122, 669-82. PMID: 16125763 4. Kawai, T. et al. (2005) Nature immunology 6, 981-8. PMID: 16127453 5. Xu, LG. et al. (2005) Molecular cell 19, 727-40. PMID: 16153868 6. Saito, T. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 582-7. PMID: 17190814 7. Friedman, CS. et al. (2008) EMBO reports 9, 930-6. PMID: 18636086 8. Mukherjee, A. et al. (2009) The Journal of biological chemistry 284, 6486-94. PMID: 19122199 9. Bamming, D. et al. (2009) The Journal of biological chemistry 284, 9700-12. PMID: 19211564 10. Cui, J. et al. (2014) Cell research 24, 400-16. PMID: 24366338 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human DDX58 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
