

| Каталожный номер | HT380022 |
|---|---|
| Описание |
Recombinant Human DEFB4A Protein, N-GST & C-His (HT380022) expressed in E. coli, spanning Gly24-Pro64. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | O15263 |
| Длина белка | Gly24-Pro64 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 32.52 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | BD-2, Beta-defensin 2, DEFB102, DEFB2, DEFB4, DEFB4A, Defensin beta 4A, Defensin, beta 2, SAP1, Skin-antimicrobial peptide 1, hBD-2 |
| Фон | Defensin beta 4A is a ~7 kDa protein. Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria. Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM. Also exhibits antimicrobial activity against the yeast C.albicans. Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death. Acts as a ligand for C-C chemokine receptor CCR6. 1. Harder, J. et al. (2000) American journal of respiratory cell and molecular biology 22, 714-21. PMID: 10837369 2. Harder, J. et al. (1997) Nature 387, 861. PMID: 9202117 3. Järvå, M. et al. (2018) Science advances 4, eaat0979. PMID: 30050988 4. Yang, D. et al. (1999) Science (New York, N.Y.) 286, 525-8. PMID: 10521347 5. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036 |
| Примечание | For research use only |
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