Human DGAT1 Recombinant Protein (N-His) (HF844012)

Name: Human DGAT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF844012
Price: 211 USD
Availability: InStock

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Aviso · Catálogo HF844012 ha sido descontinuado.

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Vista general

Número de catálogo	HF844012
Descripción	Recombinant Human DGAT1 Protein, N-His (HF844012) expressed in E. coli, spanning Ala180-Arg427. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O75907
Longitud de proteína	Ala180-Arg427
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	31.84 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	ARAT, ACAT-related gene product 1, Diglyceride acyltransferase, Diacylglycerol O-acyltransferase 1, DGAT1, Retinol O-fatty-acyltransferase, Acyl-CoA retinol O-fatty-acyltransferase, AGRP1, DGAT
Fondo	Diacylglycerol O-acyltransferase 1 (DGAT1) is a ~55 kDa protein. Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly. 1. Orland, MD. et al. (2005) Biochimica et biophysica acta 1737, 76-82. PMID: 16214399 2. Cheng, D. et al. (2008) The Journal of biological chemistry 283, 29802-11. PMID: 18768481 3. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705 4. Wang, L. et al. (2020) Nature 581, 329-332. PMID: 32433610 5. Sui, X. et al. (2020) Nature 581, 323-328. PMID: 32433611 6. Oelkers, P. et al. (1998) The Journal of biological chemistry 273, 26765-71. PMID: 9756920
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human DGAT1 Protein, N-His

Referencias

Datasheet

Human DGAT1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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