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Human DGUOK Recombinant Protein (N-His) (HD865012)

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Aperçu
Numéro de catalogueHD865012
Description
Recombinant Human DGUOK Protein, N-His (HD865012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ16854
Longueur de protéineArg40-Leu277
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsDGK, DGUOK, Deoxyadenosine kinase, mitochondrial, Deoxyguanosine kinase, mitochondrial, EC:2.7.1.113, EC:2.7.1.76
Contexte

Deoxyguanosine kinase, mitochondrial is a ~32 kDa protein. Phosphorylates deoxyguanosine and deoxyadenosine in the mitochondrial matrix, with the highest efficiency for deoxyguanosine. In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on DGUOK and TK2. Phosphorylates certain nucleoside analogs. Widely used as target of antiviral and chemotherapeutic agents.

1. Saada, A. et al. (2001) Nature genetics 29, 342-4. PMID: 11687801
2. Mousson de Camaret, B. et al. (2007) The Biochemical journal 402, 377-85. PMID: 17073823
3. Ronchi, D. et al. (2012) Brain : a journal of neurology 135, 3404-15. PMID: 23043144
4. Johansson, M. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 7258-62. PMID: 8692979
5. Wang, L. et al. (1996) FEBS letters 390, 39-43. PMID: 8706825
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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