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| カタログ番号 | HT458012 |
|---|---|
| 説明 |
Recombinant Human DHX15 Protein, N-His (HT458012) expressed in E. coli, spanning Asp568-Tyr795. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O43143 |
| タンパク質長 | Asp568-Tyr795 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 29.13 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DDX15, DEAH box protein 15, Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, DHX15, DBP1, ATP-dependent RNA helicase #46 |
| 背景 | ATP-dependent RNA helicase DHX15 is a ~90 kDa protein. RNA helicase involved in mRNA processing and antiviral innate immunity. Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. Plays a key role in antiviral innate immunity by promoting both MAVS-dependent signaling and NLRP6 inflammasome. Acts as an RNA virus sensor: recognizes and binds viral double stranded RNA (dsRNA) and activates the MAVS-dependent signaling to produce interferon-beta and interferon lambda-3 (IFNL3). 1. Yoshimoto, R. et al. (2009) Nucleic acids research 37, 891-902. PMID: 19103666 2. Lin, ML. et al. (2009) Cancer science 100, 1443-50. PMID: 19432882 3. Mosallanejad, K. et al. (2014) Science signaling 7, ra40. PMID: 24782566 4. Lu, H. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 1364-72. PMID: 24990078 5. Studer, MK. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 7159-7170. PMID: 32179686 6. Xing, J. et al. (2021) Cell reports 35, 109205. PMID: 34161762 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human DHX15 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
