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| カタログ番号 | HD135012 |
|---|---|
| 説明 |
Recombinant Human DNAJC10 Protein, N-His (HD135012) expressed in E. coli, spanning Ser354-Asn555. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8IXB1 |
| タンパク質長 | Ser354-Asn555 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 25.47 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DNAJC10, DnaJ homolog subfamily C member 10, EC:1.8.4.-, ER-resident protein ERdj5, ERDJ5, ERdj5, Endoplasmic reticulum DNA J domain-containing protein 5, MTHr, Macrothioredoxin |
| 背景 | Endoplasmic reticulum disulfide reductase DNAJC10 is a ~91 kDa protein. Endoplasmic reticulum disulfide reductase that collaborates directly with the chaperone BIP/HSPA5 (GRP78) to maintain protein quality control by facilitating either the correct folding or the targeted degradation of misfolded proteins. Essential for efficient maturation of newly synthesized polypeptides in the endoplasmic reticulum, binds to substrate proteins and specifically catalyzes the reduction and removal of improper (non-native) disulfide bonds during the folding process. In endoplasmic reticulum-associated degradation (ERAD), DNAJC10 reduces incorrect disulfide bonds specifically in misfolded glycoproteins that have been recognized by EDEM1, a key component of the ERAD pathway, thereby enabling their retrotranslocation and degradation. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress. 1. Cunnea, PM. et al. (2003) The Journal of biological chemistry 278, 1059-66. PMID: 12411443 2. Oka, OB. et al. (2013) Molecular cell 50, 793-804. PMID: 23769672 3. Cai, X. et al. (2023) The Journal of biological chemistry 299, 105274. PMID: 37739037 4. Dong, M. et al. (2008) Molecular biology of the cell 19, 2620-30. PMID: 18400946 5. Thomas, CG. et al. (2009) The Journal of biological chemistry 284, 6282-90. PMID: 19122239 |
| 注意事項 | For research use only |
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