Human DNASE1L3 Recombinant Protein (N-His) (HB541012)

Name: Human DNASE1L3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB541012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB541012
Description	Recombinant Human DNASE1L3 Protein, N-His (HB541012) expressed in E. coli, spanning Met21-Ser305. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q13609
Longueur de protéine	Met21-Ser305
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	35.52 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Deoxyribonuclease gamma; DNase gamma; 3.1.21.-; DNase I homolog protein DHP2; Deoxyribonuclease I-like 3; DNase I-like 3; Liver and spleen DNase; LS-DNase; LSD; DNASE1L3; DHP2; DNAS1L3
Contexte	Deoxyribonuclease gamma is a ~35 kDa protein. Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. 1. Yakovlev, AG. et al. (2000) The Journal of biological chemistry 275, 21302-8. PMID: 10807908 2. Shiokawa, D. et al. (1998) Apoptosis : an international journal on programmed cell death 3, 89-95. PMID: 14646506 3. Sisirak, V. et al. (2016) Cell 166, 88-101. PMID: 27293190 4. Zeng, Z. et al. (1997) Biochemical and biophysical research communications 231, 499-504. PMID: 9070308 5. Baron, WF. et al. (1998) Gene 215, 291-301. PMID: 9714828 6. Errami, Y. et al. (2013) The Journal of biological chemistry 288, 3460-8. PMID: 23229555 7. Mizuta, R. et al. (2013) PloS one 8, e80223. PMID: 24312463
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human DNASE1L3 Protein, N-His

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Human DNASE1L3 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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