Human Desmoglein-2 Recombinant Protein (N-His) (HC461012)

Name: Human Desmoglein-2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC461012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HC461012
Description	Recombinant Human DSG2 Protein, N-His (HC461012) expressed in E. coli, spanning Lys275-Val533. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q14126
Longueur de protéine	Lys275-Val533
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	31.25 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CDHF5, HDGC, Desmoglein-2, DSG2, Cadherin family member 5
Contexte	Desmoglein-2 (DSG2) is a ~122 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Required for proliferation and viability of embryonic stem cells in the blastocyst, thereby crucial for progression of post-implantation embryonic development. Maintains pluripotency by regulating epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via interacting with and sequestering CTNNB1 to sites of cell-cell contact, thereby reducing translocation of CTNNB1 to the nucleus and subsequent transcription of CTNNB1/TCF-target genes. Promotes pluripotency and the multi-lineage differentiation potential of hematopoietic stem cells. 1. Cirillo, N. et al. (2008) Journal of cellular biochemistry 103, 598-606. PMID: 17559062 2. Xu, T. et al. (2024) Kidney international 105, 1035-1048. PMID: 38395410 3. Park, J. et al. (2018) Stem cell reports 11, 115-127. PMID: 29910125 4. Ebert, LM. et al. (2016) Angiogenesis 19, 463-86. PMID: 27338829 5. Overmiller, AM. et al. (2016) Oncotarget 7, 37536-37555. PMID: 26918609
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human DSG2 Protein, N-His

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Human Desmoglein-2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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V₂ (Working Vol.)

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