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| カタログ番号 | HD385012 |
|---|---|
| 説明 |
Recombinant Human DTX3L Protein, N-His (HD385012) expressed in E. coli, spanning Ser233-Gly510. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8TDB6 |
| タンパク質長 | Ser233-Gly510 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 34.16 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | B-lymphoma- and BAL-associated protein, BBAP, DTX3L, E3 ubiquitin-protein ligase DTX3L, EC:2.3.2.27, Protein deltex-3-like, RING-type E3 ubiquitin transferase DTX3L, Rhysin-2, Rhysin2 |
| 背景 | E3 ubiquitin-protein ligase DTX3L is a ~83 kDa protein. E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4. In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. 1. Takeyama, K. et al. (2003) The Journal of biological chemistry 278, 21930-7. PMID: 12670957 2. Yan, Q. et al. (2009) Molecular cell 36, 110-20. PMID: 19818714 3. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272 4. Zhang, Y. et al. (2015) Nature immunology 16, 1215-27. PMID: 26479788 5. Yang, CS. et al. (2017) Molecular cell 66, 503-516.e5. PMID: 28525742 6. Holleman, J. et al. (2014) Molecular biology of the cell 25, 1892-904. PMID: 24790097 |
| 注意事項 | For research use only |
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