Human DYRK1A Recombinant Protein (N-His) (HD205012)

Name: Human DYRK1A Recombinant Protein (N-His)
Brand: abinScience
SKU: HD205012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HD205012
Description	Recombinant Human DYRK1A Protein, N-His (HD205012) expressed in E. coli, spanning Gly152-Lys480. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q13627
Longueur de protéine	Gly152-Lys480
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	40.42 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	DYRK, DYRK1A, Dual specificity YAK1-related kinase, Dual specificity tyrosine-phosphorylation-regulated kinase 1A, EC:2.7.11.23, EC:2.7.12.1, HP86, MNB, MNBH, Protein kinase minibrain homolog, hMNB
Contexte	Dual specificity tyrosine-phosphorylation-regulated kinase 1A is a ~85 kDa protein. Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Plays an important role in double-strand breaks (DSBs) repair following DNA damage. Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A. 1. Ogawa, Y. et al. (2010) Nature communications 1, 86. PMID: 20981014 2. Papadopoulos, C. et al. (2011) The Journal of biological chemistry 286, 5494-505. PMID: 21127067 3. Soundararajan, M. et al. (2013) Structure (London, England : 1993) 21, 986-96. PMID: 23665168 4. Menon, VR. et al. (2019) Cell cycle (Georgetown, Tex.) 18, 531-551. PMID: 30773093 5. Shindoh, N. et al. (1996) Biochemical and biophysical research communications 225, 92-9. PMID: 8769099 6. Guard, SE. et al. (2019) Scientific reports 9, 6539. PMID: 31024071 7. Di Vona, C. et al. (2015) Molecular cell 57, 506-20. PMID: 25620562 8. Lu, H. et al. (2018) Nature 558, 318-323. PMID: 29849146
Note	For research use only

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Human DYRK1A Recombinant Protein (N-His).pdf

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