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| カタログ番号 | HD205012 |
|---|---|
| 説明 |
Recombinant Human DYRK1A Protein, N-His (HD205012) expressed in E. coli, spanning Gly152-Lys480. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13627 |
| タンパク質長 | Gly152-Lys480 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 40.42 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DYRK, DYRK1A, Dual specificity YAK1-related kinase, Dual specificity tyrosine-phosphorylation-regulated kinase 1A, EC:2.7.11.23, EC:2.7.12.1, HP86, MNB, MNBH, Protein kinase minibrain homolog, hMNB |
| 背景 | Dual specificity tyrosine-phosphorylation-regulated kinase 1A is a ~85 kDa protein. Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Plays an important role in double-strand breaks (DSBs) repair following DNA damage. Mechanistically, phosphorylates RNF169 and increases its ability to block accumulation of TP53BP1 at the DSB sites thereby promoting homologous recombination repair (HRR). Also acts as a positive regulator of transcription by acting as a CTD kinase that mediates phosphorylation of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A. 1. Ogawa, Y. et al. (2010) Nature communications 1, 86. PMID: 20981014 2. Papadopoulos, C. et al. (2011) The Journal of biological chemistry 286, 5494-505. PMID: 21127067 3. Soundararajan, M. et al. (2013) Structure (London, England : 1993) 21, 986-96. PMID: 23665168 4. Menon, VR. et al. (2019) Cell cycle (Georgetown, Tex.) 18, 531-551. PMID: 30773093 5. Shindoh, N. et al. (1996) Biochemical and biophysical research communications 225, 92-9. PMID: 8769099 6. Guard, SE. et al. (2019) Scientific reports 9, 6539. PMID: 31024071 7. Di Vona, C. et al. (2015) Molecular cell 57, 506-20. PMID: 25620562 8. Lu, H. et al. (2018) Nature 558, 318-323. PMID: 29849146 |
| 注意事項 | For research use only |
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