Human ECD Recombinant Protein (N-His) (HF615012)

Name: Human ECD Recombinant Protein (N-His)
Brand: abinScience
SKU: HF615012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HF615012
Description	Recombinant Human ECD Protein, N-His (HF615012) expressed in E. coli, spanning Met10-Asn406. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O95905
Longueur de protéine	Met10-Asn406
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	47.62 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD
Contexte	Protein ecdysoneless homolog is a ~72 kDa protein. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. 1. Zhang, Y. et al. (2006) Cancer research 66, 7167-75. PMID: 16849563 2. Suh, HW. et al. (2013) Biochemical and biophysical research communications 438, 264-9. PMID: 23880345 3. Kim, JH. et al. (2010) Biological chemistry 391, 9-19. PMID: 19919181 4. Sato, T. et al. (1999) Molecular & general genetics : MGG 260, 535-40. PMID: 9928932 5. Kim, JH. et al. (2009) The Journal of biological chemistry 284, 26402-10. PMID: 19640839 6. Mir, RA. et al. (2015) Molecular and cellular biology 36, 886-99. PMID: 26711270 7. Claudius, AK. et al. (2014) PLoS genetics 10, e1004287. PMID: 24722212 8. Saleem, I. et al. (2021) Molecular and cellular biology 41, e0010321. PMID: 33941617
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ECD Protein, N-His

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Human ECD Recombinant Protein (N-His).pdf

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Formula

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