Human EEFSEC Recombinant Protein (N-His) (HD100012)

Name: Human EEFSEC Recombinant Protein (N-His)
Brand: abinScience
SKU: HD100012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HD100012
Описание	Recombinant Human EEFSEC Protein, N-His (HD100012) expressed in E. coli, spanning Met1-Ala305. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P57772
Длина белка	Met1-Ala305
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	34.63 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	EC:3.6.5.-, EEFSEC, Elongation factor sec, Eukaryotic elongation factor, selenocysteine-tRNA-specific, SELB, Selenocysteine-specific elongation factor
Фон	Selenocysteine-specific elongation factor is a ~65 kDa protein. Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons. Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur. 1. Dobosz-Bartoszek, M. et al. (2016) Nature communications 7, 12941. PMID: 27708257 2. Hilal, T. et al. (2022) Science (New York, N.Y.) 376, 1338-1343. PMID: 35709277
Примечание	For research use only

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Human EEFSEC Recombinant Protein (N-His).pdf

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