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| カタログ番号 | HP528012 |
|---|---|
| 説明 |
Recombinant Human EGLN2/EIT6 Protein, N-His (HP528012) expressed in E. coli, spanning Glu167-Thr407. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96KS0 |
| タンパク質長 | Glu167-Thr407 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 28.95 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Estrogen-induced tag 6, EIT-6, HIF-prolyl hydroxylase 1, HIF-PH1, HPH-3, HPH-1, EIT6, PHD1, Prolyl hydroxylase domain-containing protein 1, Egl nine homolog 2, Prolyl hydroxylase EGLN2, Hypoxia-inducible factor prolyl hydroxylase 1, EGLN2 |
| 背景 | Prolyl hydroxylase EGLN2 (EIT6) is a ~43 kDa protein. Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A. Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. 1. Epstein, AC. et al. (2001) Cell 107, 43-54. PMID: 11595184 2. McNeill, LA. et al. (2002) Bioorganic & medicinal chemistry letters 12, 1547-50. PMID: 12039559 3. Cobb, RR. et al. (2005) Protein expression and purification 42, 295-304. PMID: 15925519 4. Tian, YM. et al. (2006) The Biochemical journal 397, 179-86. PMID: 16509823 5. Cummins, EP. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 18154-9. PMID: 17114296 6. Moser, SC. et al. (2013) Developmental cell 26, 381-92. PMID: 23932902 7. Huang, J. et al. (2002) The Journal of biological chemistry 277, 39792-800. PMID: 12181324 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human EGLN2/EIT6 Protein, N-His
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