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Human EHD3 Recombinant Protein (N-His) (HC245012)

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Vista general
Número de catálogoHC245012
Descripción
Recombinant Human EHD3 Protein, N-His (HC245012) expressed in E. coli, spanning Thr20-Ser402. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoQ9NZN3
Longitud de proteínaThr20-Ser402
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 46.24 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosEH domain-containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3
Fondo

EH domain-containing protein 3 is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity. Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport.

1. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250
2. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528
3. Naslavsky, N. et al. (2006) Molecular biology of the cell 17, 163-77. PMID: 16251358
4. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914
5. Naslavsky, N. et al. (2009) Journal of cell science 122, 389-400. PMID: 19139087
6. Waxmonsky, NC. et al. (2013) Journal of cell science 126, 3593-601. PMID: 23781025
7. Kotowski, SJ. et al. (2011) Neuron 71, 278-90. PMID: 21791287
8. Gudmundsson, H. et al. (2010) Circulation research 107, 84-95. PMID: 20489164
Nota For research use only
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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