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| カタログ番号 | HD027012 |
|---|---|
| 説明 |
Recombinant Human EIF1 Protein, C-His (HD027012) expressed in E. coli, spanning Met1-Phe113. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P41567 |
| タンパク質長 | Met1-Phe113 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 13.80 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | A121, EIF1, Eukaryotic translation initiation factor 1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1, eIF1 |
| 背景 | Eukaryotic translation initiation factor 1 is a ~12 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. Together with eIF1A (EIF1AX), EIF1 facilitates scanning and is essential for start codon recognition on the basis of AUG nucleotide context and location relative to the 5'-cap. Participates to initiation codon selection by influencing the conformation of the 40S ribosomal subunit and the positions of bound mRNA and initiator tRNA; this is possible after its binding to the interface surface of the platform of the 40S ribosomal subunit close to the P-site. Together with eIF1A (EIF1AX), also regulates the opening and closing of the mRNA binding channel, which ensures mRNA recruitment, scanning and the fidelity of initiation codon selection. Continuously monitors and protects against premature and partial base-pairing of codons in the 5'-UTR with the anticodon of initiator tRNA. 1. Pestova, TV. et al. (2002) Genes & development 16, 2906-22. PMID: 12435632 2. Lomakin, IB. et al. (2003) Genes & development 17, 2786-97. PMID: 14600024 3. Pestova, TV. et al. (1998) Nature 394, 854-9. PMID: 9732867 4. Haimov, O. et al. (2018) Molecular and cellular biology 38. PMID: 29987188 5. Luna, RE. et al. (2013) Biochemistry 52, 9510-8. PMID: 24319994 6. Luna, RE. et al. (2012) Cell reports 1, 689-702. PMID: 22813744 |
| 注意事項 | For research use only |
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