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| カタログ番号 | HC773012 |
|---|---|
| 説明 |
Recombinant Human EIF2AK4 Protein, N-His (HC773012) expressed in E. coli, spanning Pro788-Thr1020. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9P2K8 |
| タンパク質長 | Pro788-Thr1020 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 28.78 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:2.7.11.1, EIF2AK4, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2, GCN2-like protein, KIAA1338, eIF-2-alpha kinase GCN2 |
| 背景 | eIF-2-alpha kinase GCN2 is a ~186 kDa protein. Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs. Required for the translational induction of protein kinase PRKCH following amino acid starvation. 1. Roobol, A. et al. (2015) The Biochemical journal 465, 213-25. PMID: 25329545 2. Wu, CC. et al. (2020) Cell 182, 404-416.e14. PMID: 32610081 3. Lehman, SL. et al. (2015) PLoS genetics 11, e1005212. PMID: 26102367 |
| 注意事項 | For research use only |
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