Human EIF2B5 Recombinant Protein (N-His) (HD192012)

Name: Human EIF2B5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD192012
Price: 211 USD
Availability: InStock

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Número de catálogo	HD192012
Descripción	Recombinant Human EIF2B5 Protein, N-His (HD192012) expressed in E. coli, spanning Pro42-Gly516. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q13144
Longitud de proteína	Pro42-Gly516
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	55.32 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	EIF2B5, EIF2BE, Translation initiation factor eIF2B subunit epsilon, eIF2B GDP-GTP exchange factor subunit epsilon
Fondo	Translation initiation factor eIF2B subunit epsilon is a ~80 kDa protein. Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. 1. Sekine, Y. et al. (2015) Science (New York, N.Y.) 348, 1027-30. PMID: 25858979 2. Kashiwagi, K. et al. (2016) Journal of structural and functional genomics 17, 33-8. PMID: 27023709 3. Kashiwagi, K. et al. (2019) Science (New York, N.Y.) 364, 495-499. PMID: 31048492
Nota	For research use only

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Referencias

Datasheet

Human EIF2B5 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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