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| カタログ番号 | HC232012 |
|---|---|
| 説明 |
Recombinant Human EIF3A Protein, N-His (HC232012) expressed in E. coli, spanning Met1-Arg520. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q14152 |
| タンパク質長 | Met1-Arg520 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 63.05 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EIF3A, EIF3S10, Eukaryotic translation initiation factor 3 subunit 10, Eukaryotic translation initiation factor 3 subunit A, KIAA0139, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185, eIF3a |
| 背景 | Eukaryotic translation initiation factor 3 subunit A is a ~166 kDa protein. RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. 1. Masutani, M. et al. (2007) The EMBO journal 26, 3373-83. PMID: 17581632 2. Lee, AS. et al. (2015) Nature 522, 111-4. PMID: 25849773 3. Lin, L. et al. (2001) Journal of cellular biochemistry 80, 483-90. PMID: 11169732 4. Lee, AS. et al. (2016) Nature 536, 96-9. PMID: 27462815 |
| 注意事項 | For research use only |
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