Human EIF5 Recombinant Protein (N-His) (HD092012)

Name: Human EIF5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD092012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HD092012
Описание	Recombinant Human EIF5 Protein, N-His (HD092012) expressed in E. coli, spanning Leu228-Glu386. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P55010
Длина белка	Leu228-Glu386
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	21.06 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	EIF5, Eukaryotic translation initiation factor 5, eIF-5
Фон	Eukaryotic translation initiation factor 5 is a ~49 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC. When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP. 1. Paulin, FE. et al. (2001) Current biology : CB 11, 55-9. PMID: 11166181 2. Luna, RE. et al. (2012) Cell reports 1, 689-702. PMID: 22813744 3. Luna, RE. et al. (2013) Biochemistry 52, 9510-8. PMID: 24319994
Примечание	For research use only

Изображения

Ссылки

Даташит

Human EIF5 Recombinant Protein (N-His).pdf

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