Human EIF5B Recombinant Protein (N-GST) (HC158012)

Name: Human EIF5B Recombinant Protein (N-GST)
Brand: abinScience
SKU: HC158012
Price: 211 USD
Availability: InStock

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Número de catálogo	HC158012
Descripción	Recombinant Human EIF5B Protein, N-GST (HC158012) expressed in E. coli, spanning Thr871-Ile1220. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O60841
Longitud de proteína	Thr871-Ile1220
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	66.38 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	EC:3.6.5.3, EIF5B, Eukaryotic translation initiation factor 5B, IF2, KIAA0741, Translation initiation factor IF-2, eIF-5B
Fondo	Eukaryotic translation initiation factor 5B is a ~138 kDa protein. Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. 1. Pestova, TV. et al. (2000) Nature 403, 332-5. PMID: 10659855 2. Lapointe, CP. et al. (2022) Nature 607, 185-190. PMID: 35732735 3. Marintchev, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1535-40. PMID: 12569173
Nota	For research use only

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Datasheet

Human EIF5B Recombinant Protein (N-GST).pdf

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Formula

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