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| カタログ番号 | HC158012 |
|---|---|
| 説明 |
Recombinant Human EIF5B Protein, N-GST (HC158012) expressed in E. coli, spanning Thr871-Ile1220. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O60841 |
| タンパク質長 | Thr871-Ile1220 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 66.38 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:3.6.5.3, EIF5B, Eukaryotic translation initiation factor 5B, IF2, KIAA0741, Translation initiation factor IF-2, eIF-5B |
| 背景 | Eukaryotic translation initiation factor 5B is a ~138 kDa protein. Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. 1. Pestova, TV. et al. (2000) Nature 403, 332-5. PMID: 10659855 2. Lapointe, CP. et al. (2022) Nature 607, 185-190. PMID: 35732735 3. Marintchev, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1535-40. PMID: 12569173 |
| 注意事項 | For research use only |
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