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| カタログ番号 | HC510022 |
|---|---|
| 説明 |
Recombinant Human EP300 Protein, N-His (HC510022) expressed in E. coli, spanning Glu1351-Ile1601. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q09472 |
| タンパク質長 | Glu1351-Ile1601 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 31.76 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Histone crotonyltransferase p300, Protein lactyltransferas p300, Protein propionyltransferase p300, Histone acetyltransferase p300, Protein 2-hydroxyisobutyryltransferase p300, EP300, p300 HAT, Histone butyryltransferase p300, P300, E1A-associated protein p300 |
| 背景 | Histone acetyltransferase p300 (EP300) is a ~264 kDa protein. Functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac, respectively). 1. Tropberger, P. et al. (2013) Cell 152, 859-72. PMID: 23415232 2. Delvecchio, M. et al. (2013) Nature structural & molecular biology 20, 1040-6. PMID: 23934153 3. Tian, Y. et al. (2025) Nature 641, 779-787. PMID: 40240600 4. Ogryzko, VV. et al. (1996) Cell 87, 953-9. PMID: 8945521 5. Jin, Q. et al. (2011) The EMBO journal 30, 249-62. PMID: 21131905 6. Hatzi, K. et al. (2013) Cell reports 4, 578-88. PMID: 23911289 8. Iioka, T. et al. (2003) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 18, 1419-29. PMID: 12929931 9. Yuan, ZL. et al. (2005) Science (New York, N.Y.) 307, 269-73. PMID: 15653507 10. Ray, S. et al. (2005) Gastroenterology 129, 1616-32. PMID: 16285960 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human EP300 Protein, N-His
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