ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
17.33 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CAAX prenyl protease 1 homolog (FACE1/ZMPSTE24) is a ~54 kDa protein. Transmembrane metalloprotease whose catalytic activity is critical for processing lamin A/LMNA on the inner nuclear membrane and clearing clogged translocons on the endoplasmic reticulum. Proteolytically removes the C-terminal three residues of farnesylated proteins. Also plays an antiviral role independently of its protease activity by restricting enveloped RNA and DNA viruses, including influenza A, Zika, Ebola, Sindbis, vesicular stomatitis, cowpox, and vaccinia. Mechanistically, controls IFITM antiviral pathway to hinder viruses from breaching the endosomal barrier by modulating membrane fluidity.
1. Babatz, TD. et al. (2021) The Journal of biological chemistry 296, 100165. PMID: 33293369
2. Wood, KM. et al. (2020) PloS one 15, e0239269. PMID: 33315887
3. Wang, L. et al. (2017) Nature communications 8, 13876. PMID: 28169297
4. Fu, B. et al. (2017) The Journal of experimental medicine 214, 919-929. PMID: 28246125
5. Stott-Marshall, RJ. et al. (2022) Frontiers in microbiology 13, 840885. PMID: 35283811
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human FACE1/ZMPSTE24 Protein, N-His
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