Human FBL Recombinant Protein (N-His) (HB963012)

Name: Human FBL Recombinant Protein (N-His)
Brand: abinScience
SKU: HB963012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Обзор

Каталожный номер	HB963012
Описание	Recombinant Human FBL Protein, N-His (HB963012) expressed in E. coli, spanning Gly37-Ala275. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P22087
Длина белка	Gly37-Ala275
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	27.33 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	FBL, Histone-glutamine methyltransferase, 34 kDa nucleolar scleroderma antigen, FIB1, rRNA 2'-O-methyltransferase fibrillarin, U6 snRNA 2'-O-methyltransferase fibrillarin, FLRN
Фон	rRNA 2'-O-methyltransferase fibrillarin (FBL) is a ~33 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. 1. Tessarz, P. et al. (2014) Nature 505, 564-8. PMID: 24352239 2. Iyer-Bierhoff, A. et al. (2018) Cell reports 25, 2946-2954.e5. PMID: 30540930 3. Hasler, D. et al. (2020) Molecular cell 77, 1014-1031.e13. PMID: 32017898 4. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human FBL Protein, N-His

Ссылки

Даташит

Human FBL Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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