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| カタログ番号 | HD309012 |
|---|---|
| 説明 |
Recombinant Human FBXO31 Protein, N-His (HD309012) expressed in E. coli, spanning Pro71-Ile289. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q5XUX0 |
| タンパク質長 | Pro71-Ile289 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 28.31 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | F-box only protein 31, FBX14, FBX31, FBXO31 |
| 背景 | F-box only protein 31 is a ~60 kDa protein. Substrate-recognition component of the SCF(FBXO31) protein ligase complex, which specifically mediates the ubiquitination of proteins amidated at their C-terminus in response to oxidative stress, leading to their degradation by the proteasome. FBXO31 specifically recognizes and binds C-terminal peptides bearing an amide: C-terminal amidation in response to oxidative stress takes place following protein fragmentation. The SCF(FBXO31) also plays a role in G1 arrest following DNA damage by mediating ubiquitination of phosphorylated cyclin-D1 (CCND1), promoting its degradation by the proteasome, resulting in G1 arrest. The SCF(FBXO31) complex is however not a major regulator of CCND1 stability during the G1/S transition. In response to genotoxic stress, the SCF(FBXO31) complex directs ubiquitination and degradation of phosphorylated MDM2, thereby promoting p53/TP53-mediated DNA damage response. 1. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951 2. Santra, MK. et al. (2009) Nature 459, 722-5. PMID: 19412162 3. Li, Y. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 319-324. PMID: 29279382 4. Malonia, SK. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8632-7. PMID: 26124108 5. Dutta, P. et al. (2019) The Journal of biological chemistry 294, 14879-14895. PMID: 31413110 6. Liu, J. et al. (2014) The Journal of biological chemistry 289, 21508-18. PMID: 24936062 7. Choppara, S. et al. (2018) The Journal of biological chemistry 293, 16291-16306. PMID: 30171069 8. Johansson, P. et al. (2014) The Journal of biological chemistry 289, 18514-25. PMID: 24828503 9. Vadhvani, M. et al. (2013) PloS one 8, e57530. PMID: 23469015 |
| 注意事項 | For research use only |
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