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| カタログ番号 | HC898012 |
|---|---|
| 説明 |
Recombinant Human FFAR2 Protein, N-GST & C-His (HC898012) expressed in E. coli, spanning Ser277-Glu330. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O15552 |
| タンパク質長 | Ser277-Glu330 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.91 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | FFA2, FFAR2, Free fatty acid receptor 2, G-protein coupled receptor 43, GPCR43, GPR43 |
| 背景 | Free fatty acid receptor 2 is a ~37 kDa protein. G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family. Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. 1. Brown, AJ. et al. (2003) The Journal of biological chemistry 278, 11312-9. PMID: 12496283 2. Le Poul, E. et al. (2003) The Journal of biological chemistry 278, 25481-9. PMID: 12711604 3. Hudson, BD. et al. (2013) The Journal of biological chemistry 288, 17296-312. PMID: 23589301 4. Zhang, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 6899-910. PMID: 21037097 5. Hudson, BD. et al. (2012) The Journal of biological chemistry 287, 41195-209. PMID: 23066016 |
| 注意事項 | For research use only |
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