中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HP668011 |
|---|---|
| 説明 |
Recombinant Human FTO Protein, C-His (HP668011) expressed in Mammalian Cells, spanning Met1-Pro505. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | Q9C0B1 |
| タンパク質長 | Met1-Pro505 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, 1.14.11.-, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, 1.14.11.-, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, m6A(m)-demethylase FTO, 1.14.11.-, mRNA N(6)-methyladenosine demethylase FTO, 1.14.11.53, tRNA N1-methyl adenine demethylase FTO, 1.14.11.-, FTO, KIAA1752 |
| 背景 | Alpha-ketoglutarate-dependent dioxygenase FTO is a ~58 kDa protein. RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Jia, G. et al. (2011) Nature chemical biology 7, 885-7. PMID: 22002720 _x005F_x005F_x000D_
_x005F_x005F_x000D_
2. Huang, Y. et al. (2015) Nucleic acids research 43, 373-84. PMID: 25452335 _x005F_x005F_x000D_
_x005F_x005F_x000D_
3. Wang, T. et al. (2015) Journal of the American Chemical Society 137, 13736-9. PMID: 26457839 _x005F_x005F_x000D_
_x005F_x005F_x000D_
4. Zhou, J. et al. (2015) Nature 526, 591-4. PMID: 26458103 _x005F_x005F_x000D_
_x005F_x005F_x000D_
5. Mauer, J. et al. (2017) Nature 541, 371-375. PMID: 28002401 _x005F_x005F_x000D_
_x005F_x005F_x000D_
6. Wei, J. et al. (2018) Molecular cell 71, 973-985.e5. PMID: 30197295 _x005F_x005F_x000D_ |
| 注意事項 | For research use only. |
SDS-PAGE for Human FTO Recombinant Protein (C-His)
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
