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| カタログ番号 | HY292011 |
|---|---|
| 説明 |
Recombinant Human GBA Protein, C-Fc (HY292011) expressed in Mammalian Cells, spanning Ala40-Gln536. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | P04062 |
| タンパク質長 | Ala40-Gln536 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 83.93 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Lysosomal acid glucosylceramidase, Lysosomal acid GCase, EC:3.2.1.45, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-GC, Beta-glucosylceramidase 1, Cholesterol glucosyltransferase, SGTase, EC:2.4.1.-, Cholesteryl-beta-glucosidase, EC:3.2.1.-, D-glucosyl-N-acylsphingosine glucohydrolase, Glucosylceramidase beta 1, Imiglucerase, Lysosomal cholesterol glycosyltransferase, Lysosomal galactosylceramidase, EC:3.2.1.46, Lysosomal glycosylceramidase, GBA1 |
| 背景 | Lysosomal acid glucosylceramidase (GBA1) is a ~59 kDa protein. Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (such as N-acylsphing-4-enine) and glucose. Plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation. Catalyzes the glucosylation of cholesterol, through a transglucosylation reaction where glucose is transferred from GlcCer to cholesterol. GlcCer containing mono-unsaturated fatty acids (such as beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred as glucose donors for cholesterol glucosylation when compared with GlcCer containing same chain length of saturated fatty acids (such as beta-D-glucosyl-N-octadecanoyl-sphing-4-enine). 1. Ron, I. et al. (2005) Blood cells, molecules & diseases 35, 57-65. PMID: 15916907 2. Akiyama, H. et al. (2013) Biochemical and biophysical research communications 441, 838-43. PMID: 24211208 3. Akiyama, H. et al. (2020) The Journal of biological chemistry 295, 5257-5277. PMID: 32144204 4. Bannink, S. et al. (2024) Journal of lipid research 65, 100670. PMID: 39395789 5. Vaccaro, AM. et al. (1997) The Journal of biological chemistry 272, 16862-7. PMID: 9201993 6. Magalhaes, J. et al. (2016) Human molecular genetics 25, 3432-3445. PMID: 27378698 7. Kitatani, K. et al. (2009) The Journal of biological chemistry 284, 12972-8. PMID: 19279011 8. Marques, AR. et al. (2016) Journal of lipid research 57, 451-63. PMID: 26724485 9. Boer, DE. et al. (2021) Journal of lipid research 62, 100018. PMID: 33361282 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human GBA1 Protein, C-Fc
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