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Обзор
Каталожный номерHD909012
Описание
Recombinant Human GFI1 Protein, N-His (HD909012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ99684
Длина белкаLys256-Lys422
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияGFI1, Growth factor independent protein 1, ZNF163, Zinc finger protein 163, Zinc finger protein Gfi-1
Фон

Zinc finger protein Gfi-1 is a ~45 kDa protein. Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development.

1. Rödel, B. et al. (2000) The EMBO journal 19, 5845-55. PMID: 11060035
2. Dahl, R. et al. (2007) The Journal of biological chemistry 282, 6473-83. PMID: 17197705
3. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546
4. Montoya-Durango, DE. et al. (2008) The Journal of biological chemistry 283, 32056-65. PMID: 18805794
5. Basu, S. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 1433-8. PMID: 19164764
6. Liu, Q. et al. (2010) Oncogene 29, 2843-52. PMID: 20190815
7. Zweidler-Mckay, PA. et al. (1996) Molecular and cellular biology 16, 4024-34. PMID: 8754800
10. Sharif-Askari, E. et al. (2010) Molecular and cellular biology 30, 3929-42. PMID: 20547752
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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