Human GNA13 Recombinant Protein (N-His) (HB520012)

Name: Human GNA13 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB520012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HB520012
Описание	Recombinant Human GNA13 Protein, N-His (HB520012) expressed in E. coli, spanning Leu48-Gln377. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q14344
Длина белка	Leu48-Gln377
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	41.03 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Guanine nucleotide-binding protein subunit alpha-13; G alpha-13; G-protein subunit alpha-13; GNA13
Фон	Guanine nucleotide-binding protein subunit alpha-13 is a ~44 kDa protein. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in a process independent from Rho activation. 1. Krakstad, BF. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 10314-9. PMID: 15240885 2. Kelly, P. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8173-8. PMID: 16705036 3. Kelly, P. et al. (2006) The Journal of biological chemistry 281, 26483-90. PMID: 16787920 4. Yuan, B. et al. (2016) Biochemical and biophysical research communications 473, 1240-1246. PMID: 27084452 5. Suzuki, N. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 733-8. PMID: 12515866 6. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human GNA13 Protein, N-His

Ссылки

Даташит

Human GNA13 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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