Human GPR4 Recombinant Protein (N-His-KSI) (HA696012)

Name: Human GPR4 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HA696012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HA696012
Описание	Recombinant Human GPR4 Protein, N-His-KSI (HA696012) expressed in E. coli, spanning Ile284-Gln362. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P46093
Длина белка	Ile284-Gln362
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	24.26 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l; GPR4
Фон	G-protein coupled receptor 4 is a ~40 kDa protein. Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. Activated by an optimal pH of 6.8-7.2. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPR4 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. May also couple with G(i), G(q) and G(12)/G(13) G proteins. 1. Ludwig, MG. et al. (2003) Nature 425, 93-8. PMID: 12955148 2. Tobo, M. et al. (2007) Cellular signalling 19, 1745-53. PMID: 17462861 3. Rowe, JB. et al. (2021) The Journal of biological chemistry 296, 100167. PMID: 33478938 4. Howard, MK. et al. (2025) Cell 188, 671-687.e20. PMID: 39753132 5. Ma, Y. et al. (2025) Nature communications 16, 605. PMID: 39799123 6. Yue, X. et al. (2025) Cell research 35, 423-436. PMID: 40211064 7. Guo, L. et al. (2025) Molecular cell 85, 1658-1673.e7. PMID: 40215959 8. Chen, LN. et al. (2025) Molecular cell 85, 1640-1657.e8. PMID: 40215960
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human GPR4 Protein, N-His-KSI

Ссылки

Даташит

Human GPR4 Recombinant Protein (N-His-KSI).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ (Stock Conc.)

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