

| Numéro de catalogue | HD836012 |
|---|---|
| Description |
Recombinant Human GTF2B Protein, N-His (HD836012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Système d'expression | E. coli |
| Numéro d'accès | Q00403 |
| Longueur de protéine | Ser108-Leu316 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Espèces | Homo sapiens (Human) |
| Nature | Recombinant |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >90% as determined by SDS-PAGE. |
| Forme | Lyophilized |
| Tampon de stockage | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Expédition | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Noms alternatifs | EC:2.3.1.48, GTF2B, General transcription factor TFIIB, S300-II, TF2B, TFIIB, Transcription initiation factor IIB |
| Contexte | Transcription initiation factor IIB is a ~34 kDa protein. General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. 1. Choi, CH. et al. (2003) Nature 424, 965-9. PMID: 12931194 2. Ing, NH. et al. (1992) The Journal of biological chemistry 267, 17617-23. PMID: 1517211 3. Ha, I. et al. (1991) Nature 352, 689-95. PMID: 1876184 4. Malik, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 9553-7. PMID: 1946368 5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682 6. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3310-21. PMID: 3029109 7. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3322-30. PMID: 3818643 8. Zawel, L. et al. (1995) Genes & development 9, 1479-90. PMID: 7601352 9. Malik, S. et al. (1993) Molecular and cellular biology 13, 6253-9. PMID: 8413225 10. Hisatake, K. et al. (1993) Nature 363, 744-7. PMID: 8515820 |
| Note | For research use only |
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