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Обзор
Каталожный номерHD836012
Описание
Recombinant Human GTF2B Protein, N-His (HD836012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ00403
Длина белкаSer108-Leu316
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияEC:2.3.1.48, GTF2B, General transcription factor TFIIB, S300-II, TF2B, TFIIB, Transcription initiation factor IIB
Фон

Transcription initiation factor IIB is a ~34 kDa protein. General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions.

1. Choi, CH. et al. (2003) Nature 424, 965-9. PMID: 12931194
2. Ing, NH. et al. (1992) The Journal of biological chemistry 267, 17617-23. PMID: 1517211
3. Ha, I. et al. (1991) Nature 352, 689-95. PMID: 1876184
4. Malik, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 9553-7. PMID: 1946368
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3310-21. PMID: 3029109
7. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3322-30. PMID: 3818643
8. Zawel, L. et al. (1995) Genes & development 9, 1479-90. PMID: 7601352
9. Malik, S. et al. (1993) Molecular and cellular biology 13, 6253-9. PMID: 8413225
10. Hisatake, K. et al. (1993) Nature 363, 744-7. PMID: 8515820
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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