Human GTPBP1 Recombinant Protein (N-His) (HA399012)

Name: Human GTPBP1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA399012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор
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Даташит
SDS

Обзор

Каталожный номер	HA399012
Описание	Recombinant Human GTPBP1 Protein, N-His (HA399012) expressed in E. coli, spanning Thr71-Ser384. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O00178
Длина белка	Thr71-Ser384
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	36.87 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	GTPBP1, GTP-binding protein 1, G-protein 1, GP-1, GP1, GTPBP1
Фон	GTP-binding protein 1 (GTPBP1) is a ~72 kDa protein. GTPase that plays a role in the elongation phase of protein synthesis by forming ternary complexes with GTP and aminoacyl-transfer RNAs (aa-tRNAs), and delivering aa-tRNAs to the ribosomal A site in a GTP-dependent manner. Is also able to deliver deacylated tRNA to the A site. Additionally, it is involved in RNA quality control; after GTP hydrolysis, which is not immediately followed by rapid peptide bond formation, GTPBP1 likely retains aa-tRNA in the A site and promotes exosomal degradation of faulty mRNAs engaged in 80S elongation complexes. Plays a role in the regulation of circadian mRNA stability. 1. Zinoviev, A. et al. (2018) Genes & development 32, 1226-1241. PMID: 30108131
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human GTPBP1 Protein, N-His

Ссылки

Даташит

Human GTPBP1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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